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Workshop Outline

°Õ³ó¾±²õÌý3-day hybridÌýworkshopÌý·É¾±±ô±ôÌýfocusÌýon topics ranging fromÌýbasic experimental designÌý³Ù´ÇÌýadvanced downstream analysesÌý´Ç´ÚÌý³¾¸é±·´¡-²õ±ð±çÌýdata as well asÌýtips to publish in high-impact journals.

Hands-on trainingÌýfrom RNA-seqÌýdataÌý³Ù´ÇÌýpublicationÌýfigures / tablesÌýwithoutÌýUNIX / Python / R scripts.
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Upon the completion of thisÌý3-day hybrid workshop, participants will be capable of the following:

  • ConceptualisingÌýa transcriptome experiment with different considerations
  • ±«²Ô»å±ð°ù²õ³Ù²¹²Ô»å¾±²Ô²µÌýa complete workflow ofÌýmRNA-seqÌýdata acquisition, processing, assembly, functional annotation, analysis, and interpretation
  • AwareÌý´Ç´Ú the different analysis workflows and software packages in RNA-seq analysis
  • CompetentÌýinÌýsubmitting RNA-seq relatedÌý´Ú¾±±ô±ð²õÌýto theÌý±·°äµþ±õÌý±è³Ü²ú±ô¾±³¦Ìýrepository
  • ExposureÌýto different approaches forÌý¾±²Ô³Ù±ð²µ°ù²¹³Ù¾±²Ô²µÌýtranscriptomics withÌýother omicsÌý³Ù´Ç·É²¹°ù»å²õÌýsystems understanding
  • Network opportunitiesÌýwith other researchers in the field &Ìýsharing of knowledgeÌývia voluntaryÌýflash talks
  • Walk-through onÌýde novoÌýtranscriptomic analysisÌý²¹²Ô»åÌý³¾¾±²Ô¾±²Ô²µÌýRNA-seq data
  • Hands-on in downstreamÌýfunctional analyses: DEG, WEGO, KEGG pathway, clustering, functional enrichment usingÌýExcel,ÌýPerseus, ExpressAnalyst, and otherÌýweb-based tools.
  • Producing publication-standard result figures

Workshop Highlights

  1. PrinciplesÌýof transcriptomics
  2. ExperimentalÌýdesignÌý&Ìýconsiderations
  3. Technical talksÌýfrom reputable local companies on the latest developments in the field
  4. Comprehensive topics, includingÌýsingle-cell RNA-seq, spatialomics,Ìý, etc.
  5. CompleteÌýworkflowÌýof transcriptomic analysis
  6. FunctionalÌýannotationÌý&ÌýenrichmentÌýanalysis
  7. Biologist-friendlyÌýapproach toÌýdownstream omics analysesÌýusingÌý,Ìý, and web-based tools
  8. Data submissionÌýto NCBI ±è³Ü²ú±ô¾±³¦Ìýrepository
  9. TipsÌýforÌýpublishingÌýtranscriptomicsÌýjournal article
  10. WorkshopÌýmaterialsÌý&Ìývideo recordingsÌýwill be available for access after the workshop
  11. Global participantsÌýwith opportunities ofÌýconsultationÌýor sharing viaÌý
  12. ​Certificate of participation

Why you should attend?

  • You're aÌýresearcher, academician, orÌýstudentÌýinterested in transcriptomics and its applications.

  • You're interested inÌýadvancedÌýskillsÌýin RNA-seq analysis.

  • You wantÌýuser-friendly platformsÌýfor the interpretation of RNA-seq data.

  • You got the transcriptomeÌýresultsÌýfrom the sequencing vendorÌýbut not sure how toÌýinterpretÌýthem.

  • You wish toÌýanalyseÌýthe transcriptome dataÌýhands-on,Ìýespecially as a biologist instead ofÌýa data scientist.

  • You want to learn on how to mine forÌýbiological knowledgeÌýfrom transcriptome dataÌýwithout programming/coding.

  • You work on a transcriptomic project and want to learn on how toÌýpublishÌýyour results as aÌýhigh-impactÌýjournal article.

This workshop is opened toÌýlocalÌý&ÌýinternationalÌýparticipants, from beginner to advanced researchersÌýinÌýmolecular biologyÌý/Ìýomics. TheÌýcontentÌý´Ç´Ú this workshop isÌýtransferableÌýto other omics studies such asÌýproteomics.

Fees

Rate Per Person

/ Mode

On-site

Online

Individual

Local : RM 900

International : RM 1,000

Local : RM 400

International : RM 450

Group ofÌý

Local : RM 750

International : RM 800

Local : RM 300

International : RM 360

Speaker Biography

  • Associate Professor Dr. Goh Hoe Han is a plant molecular biologist who obtained his PhD from the University of Sheffield, United Kingdom in 2011 before starting his first acedemic position at the Institute of Systems Biology, Universiti Kebangsaan Malaysia. Upon joining INBIOSIS, he pioneered the Plant Functional Genomics Research Group focusing on the molecular exploration of tropical plants and crop improvement using functional genomics approaches. Some of the tropical plant species include kesum, ketum, Rafflesia, and Nepenthes pitcher plants, as well as important crops, such as mangosteen, papaya, rice, tomato, and oil palm. Fundamental understandings on the molecular aspects of these plants are crucial for their bioprospecting, industrial application, and crop improvement.

  • Dr Goh is keen on industrial collaboration in precision biotechnology for tropical plant improvement and also the commercialisation of novel recombinant plant enzymes. If you are interested to collaborate or be part of the plant functional genomics group, please email to gohhh@ukm.edu.my.

Programme

17th June 2025 (Tuesday)

Time Activity
8.30 am Registration
9.00 am Introduction
9.10 am Lecture 1: Principles of transcriptomics
10.00 am Technical Talk - Current Developments of RNA-seqÌý
10.30 am Morning break
10.45 am Lecture 2: A crash course on RNA-seq analysis
12.30 pm Technical Talk - Spatial transcriptomics - STOmics
1.00 pm Lunch break
2.00 pm Practical 1 : Data pre-processing/exploration
4.00 pm Afternoon break
4.15 pm Participant flash talks, summary, discussions, and wrap-up
5.00 pm END OF DAY 1

 

18th June 2025 (Wednesday)

Time Activity
9.00 am Introduction to Day 2 & Recap
9.10 am Lecture 3: Exploring RNA-seq analysis for biological discovery (Functional Annotation, DEG & Downstream Analyses)
10.30 am Morning break
10.45 am Practical 2: Statistical Analysis
12.30 pm Technical Talk - Single-cell RNA-seq
1.00 pm Lunch break
2.00 pm Practical 3: Functional Enrichment analysis
4.o0 pm Afternoon break
4.15 pm Participant flash talks, summary, discussions, and wrap-up
5.00 pm END OF DAY 2

19th June 2025 (Thursday)

Time Activity
9.00 am Introduction to Day 3 & Recap
9.10 am Lecture 4 : RNA-seq downstream analyses
10.30 am Morning break
10.45 am Practical 4 : Cluster analysis and visualisation
12.30 pm Technical talk - qPCR validation
1.00 pm Lunch break
2.00 pm Practical 5: ExpressAnalyst
4.00 pm Afternoon break
4.15 pm Summary, discussions, and conclusion
5.00 pm END OF WORKSHOP

 

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