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°Õ³ó¾±²õÌý3-day onlineÌýworkshopÌý·É¾±±ô±ôÌýfocusÌýon topics ranging fromÌýbasic experimental designÌý³Ù´ÇÌýadvanced downstream analysesÌý´Ç´ÚÌý³¾¸é±·´¡-²õ±ð±çÌýdata as well asÌýtips to publish in high-impact journals.

Hands-on trainingÌýfrom RNA-seqÌýdataÌý³Ù´ÇÌýpublicationÌýfigures / tablesÌýwithoutÌýUNIX / Python / R scripts.
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Upon the completion of thisÌý3-day online workshop, participants will be capable of the following:

• ConceptualisingÌýa transcriptome experiment with different considerations
• ±«²Ô»å±ð°ù²õ³Ù²¹²Ô»å¾±²Ô²µÌýa complete workflow ofÌýmRNA-seqÌýdata acquisition, processing, assembly, functional annotation, analysis, and interpretation
• AwareÌý´Ç´Ú the different analysis workflows and software packages in RNA-seq analysis
• CompetentÌýinÌýsubmitting RNA-seq relatedÌý´Ú¾±±ô±ð²õÌýto ³Ù³ó±ðÌý±·°äµþ±õÌý±è³Ü²ú±ô¾±³¦Ìýrepository
• ExposureÌýto different approaches forÌý¾±²Ô³Ù±ð²µ°ù²¹³Ù¾±²Ô²µÌýtranscriptomics withÌýother omicsÌý³Ù´Ç·É²¹°ù»å²õÌýsystems understanding
• ±«²Ô»å±ð°ù²õ³Ù²¹²Ô»åÌý³Ù³ó±ðÌýapplications of AIÌýin RNA-seq analysis
• Network opportunitiesÌýwith other researchers in the field &Ìýsharing of knowledgeÌývia voluntaryÌýflash talks
• Walk-through onÌýde novoÌýtranscriptomic analysisÌý²¹²Ô»åÌý³¾¾±²Ô¾±²Ô²µÌýRNA-seq data
• Hands-on in downstreamÌýfunctional analyses: DEG, WEGO, KEGG pathway, clustering, functional enrichment usingÌýExcel,ÌýPerseus, ExpressAnalyst, and otherÌýweb-based tools.
• Producing publication-standard result figures

1. PrinciplesÌýof transcriptomics
2. ExperimentalÌýdesignÌý&Ìýconsiderations
3. Technical talksÌýfrom reputable local companies on the latest developments in the field
4. Comprehensive topics, includingÌýsingle-cell RNA-seq, spatialomics,Ìý, etc.
5. CompleteÌýworkflowÌýof transcriptomic analysis
6. FunctionalÌýannotationÌý&ÌýenrichmentÌýanalysis
7. AI applicationsÌýin RNA-seq
8. Biologist-friendlyÌýapproach toÌýdownstream omics analysesÌýusingÌý,Ìý, and web-based tools
9. Data submissionÌýto NCBI ±è³Ü²ú±ô¾±³¦Ìýrepository
10. TipsÌýforÌýpublishingÌýtranscriptomicsÌýjournal article
11. WorkshopÌýmaterialsÌý&Ìývideo recordingsÌýwill be available for access after the workshop
12. Global participantsÌýwith opportunities ofÌýconsultationÌýor sharing viaÌý
13. ​Certificate & LinkedIn Badge of completion

For online participants, please download and installÌýÌýfor the online sessions. You won't need a computer with very high specifications.

​Minimum requirements: Windows OS (ideallyÌý64-bitÌýWindows 10Ìýand above) with minimumÌý2 GB RAMÌý²¹²Ô»åÌý10 GB hard disk storageÌýto runÌýÌý(2016 and above) ²¹²Ô»åÌýsoftware for day 2 and day 3 practicals, respectively.ÌýForÌýMacÌýusers, please run Windows viaÌýParallels or Bootcamp etc.
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We will be usingÌýPerseus v2.0.9. Installers can be downloadedÌý. You just need to unzip the Perseus folder.

It's recommended that youÌýuse two devicesÌý(e.g., desktop/smartphone + laptop) orÌýdual monitor setupÌýto allow easy following ofÌýonline demosÌýduringÌýpracticals.

For hands-on practicals, make sure that all software (Excel & Perseus) can run on your laptop before the workshop.ÌýDue to time constraint we don't have the time to individually troubleshoot software installation. As aÌýpre-workshop training, you can try out thisÌýÌýwithÌýthe demoÌýÌýto get familiar with Perseus basic functions. You can refer to PerseusÌýdemo videosÌýÌý&Ìý.

Don't worry if your laptop cannot run Perseus, you can still learn a lot from this workshop on transcriptomics, different technologies, analysis workflow, applications, data interpretation, and visualisation of omics data.

• You're aÌýresearcher, academician, orÌýstudentÌýinterested in transcriptomics and its applications.

• You're interested inÌýadvancedÌýskillsÌýin RNA-seq analysis.

• You wantÌýuser-friendly platformsÌýfor the interpretation of RNA-seq data.

• You got the transcriptomeÌýresultsÌýfrom the sequencing vendorÌýbut not sure how toÌýinterpretÌýthem.

• You wish toÌýanalyseÌýthe transcriptome dataÌýhands-on,Ìýespecially as a biologist instead ofÌýa data scientist.

• You want to learn on how to mine forÌýbiological knowledgeÌýfrom transcriptome dataÌýwithout programming/coding.

• You work on a transcriptomic project and want to learn on how toÌýpublishÌýyour results as aÌýhigh-impactÌýjournal article.

This workshop is opened toÌýlocalÌý&ÌýinternationalÌýparticipants, from beginner to advanced researchersÌýinÌýmolecular biologyÌý/Ìýomics. TheÌýcontentÌý´Ç´Ú this workshop isÌýtransferableÌýto other omics studies such asÌýproteomics.

• Associate Professor Dr. Goh Hoe Han is a plant molecular biologist who obtained his PhD from the University of Sheffield, United Kingdom in 2011 before starting his first acedemic position at the Institute of Systems Biology, Universiti Kebangsaan Malaysia. Upon joining INBIOSIS, he pioneered the Plant Functional Genomics Research Group focusing on the molecular exploration of tropical plants and crop improvement using functional genomics approaches. Some of the tropical plant species include kesum, ketum, Rafflesia, and Nepenthes pitcher plants, as well as important crops, such as mangosteen, papaya, rice, tomato, and oil palm. Fundamental understandings on the molecular aspects of these plants are crucial for their bioprospecting, industrial application, and crop improvement.

• Dr Goh is keen on industrial collaboration in precision biotechnology for tropical plant improvement and also the commercialisation of novel recombinant plant enzymes. If you are interested to collaborate or be part of the plant functional genomics group, please email to gohhh@ukm.edu.my.