{"id":97068,"date":"2022-06-07T12:10:37","date_gmt":"2022-06-07T04:10:37","guid":{"rendered":"https:\/\/www.ukm.my\/umbi\/?page_id=97068"},"modified":"2022-08-09T16:08:16","modified_gmt":"2022-08-09T08:08:16","slug":"crispr2022","status":"publish","type":"page","link":"https:\/\/www.ukm.my\/umbi\/crispr2022\/","title":{"rendered":"CRISPR Genome Editing Workshop 2022"},"content":{"rendered":"<div class=\"osc-res-tab tabbable   osc-tabs-left\"><div style=\"clear:both;width: 100%;\"><ul class=\"nav osc-res-nav nav-tabs osc-tabs-left-ul\" id=\"oscitas-restabs-1-crispr2022-21167\"><li class=\"active\"><a href=\"#ert_pane1-0\" data-toggle=\"tab\">Home<\/a><\/li><li class=\"\"><a href=\"#ert_pane1-1\" data-toggle=\"tab\">Introduction<\/a><\/li><li class=\"\"><a href=\"#ert_pane1-2\" data-toggle=\"tab\">Registration<\/a><\/li><li class=\"\"><a href=\"#ert_pane1-3\" data-toggle=\"tab\">Schedule<\/a><\/li><li class=\"\"><a href=\"#ert_pane1-4\" data-toggle=\"tab\">Venue<\/a><\/li><li class=\"\"><a href=\"#ert_pane1-5\" data-toggle=\"tab\">Accommodation<\/a><\/li><li class=\"\"><a href=\"#ert_pane1-6\" data-toggle=\"tab\">Contact<\/a><\/li><\/ul><\/div><div style=\"clear:both;width: 100%;\"><ul class=\"tab-content\" id=\"oscitas-restabcontent-1-crispr2022-21167\"><li class=\"tab-pane active\" id=\"ert_pane1-0\"><\/p>\n<p><img fetchpriority=\"high\" decoding=\"async\" class=\"alignnone size-full wp-image-97191\" src=\"https:\/\/www.ukm.my\/umbi\/wp-content\/uploads\/2022\/06\/UMBI-Eposter-Crispr-Workshop-Final-1-min.png\" alt=\"\" width=\"5335\" height=\"3002\" srcset=\"https:\/\/www.ukm.my\/umbi\/wp-content\/uploads\/2022\/06\/UMBI-Eposter-Crispr-Workshop-Final-1-min.png 5335w, https:\/\/www.ukm.my\/umbi\/wp-content\/uploads\/2022\/06\/UMBI-Eposter-Crispr-Workshop-Final-1-min-300x169.png 300w, https:\/\/www.ukm.my\/umbi\/wp-content\/uploads\/2022\/06\/UMBI-Eposter-Crispr-Workshop-Final-1-min-1024x576.png 1024w, https:\/\/www.ukm.my\/umbi\/wp-content\/uploads\/2022\/06\/UMBI-Eposter-Crispr-Workshop-Final-1-min-768x432.png 768w, https:\/\/www.ukm.my\/umbi\/wp-content\/uploads\/2022\/06\/UMBI-Eposter-Crispr-Workshop-Final-1-min-1536x864.png 1536w, https:\/\/www.ukm.my\/umbi\/wp-content\/uploads\/2022\/06\/UMBI-Eposter-Crispr-Workshop-Final-1-min-2048x1152.png 2048w, https:\/\/www.ukm.my\/umbi\/wp-content\/uploads\/2022\/06\/UMBI-Eposter-Crispr-Workshop-Final-1-min-800x450.png 800w\" sizes=\"(max-width: 5335px) 100vw, 5335px\" \/><\/p>\n<p>&nbsp;<\/p>\n<h2 style=\"text-align: center;\"><b>Facilitators<\/b><\/h2>\n<p><img decoding=\"async\" class=\"alignleft wp-image-90530 size-medium\" src=\"https:\/\/www.ukm.my\/umbi\/wp-content\/uploads\/2016\/03\/fendi-umbi-225x300.jpg\" alt=\"\" width=\"225\" height=\"300\" srcset=\"https:\/\/www.ukm.my\/umbi\/wp-content\/uploads\/2016\/03\/fendi-umbi-225x300.jpg 225w, https:\/\/www.ukm.my\/umbi\/wp-content\/uploads\/2016\/03\/fendi-umbi.jpg 684w\" sizes=\"(max-width: 225px) 100vw, 225px\" \/><\/p>\n<h3><a href=\"https:\/\/www.ukm.my\/umbi\/saiful-effendi\/\" target=\"_blank\" rel=\"nofollow noopener\">Dr Saiful Effendi Syafruddin<\/a><\/h3>\n<p>Research Fellow<\/p>\n<p>Cancer Biology, Immunology &amp; Functional Genomics<\/p>\n<p>effendisy@ppukm.ukm.edu.my<\/p>\n<p>+603-91459072<\/p>\n<p>BSc. (Hons) Biotechnology (RIT, USA), MSc. Molecular Genetics (Uni of Pennsylvania, USA), PhD Medical Sciences (University of Cambridge)<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p><img decoding=\"async\" class=\"alignleft wp-image-90530 size-medium\" src=\"https:\/\/www.ukm.my\/umbi\/wp-content\/uploads\/2019\/08\/Aiman-mohtar-umbi-e1566975630897-239x300.jpg\" alt=\"\" width=\"225\" height=\"300\" \/><\/p>\n<h3><a href=\"https:\/\/www.ukm.my\/umbi\/dr-aiman\/\" target=\"_blank\" rel=\"nofollow noopener\">Dr. Mohamad Aimanuddin Mohtar<\/a><\/h3>\n<p>Research Fellow<\/p>\n<p>Cancer Biology and Functional Proteomics<\/p>\n<p>m.aimanmohtar@ppukm.ukm.edu.my<\/p>\n<p>+603-91459062<\/p>\n<p>BSc. Biotechnology (H. Hons) (USA), MSc. Molecular Biology (Uni of Pennsylvania, USA), PhD Molecular &amp; Clinical Medicine (Uni of Edinburgh, UK)<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<h2 style=\"text-align: center;\"><strong>Sponsors<\/strong><\/h2>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-97083 aligncenter\" src=\"https:\/\/www.ukm.my\/umbi\/wp-content\/uploads\/2022\/06\/Crispr2022_sponsor.png\" alt=\"\" width=\"250\" height=\"115\" \/><\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p><\/li><li class=\"tab-pane \" id=\"ert_pane1-1\"><\/p>\n<p style=\"text-align: justify;\"><b>Introduction<\/b><\/p>\n<p style=\"text-align: justify;\"><span style=\"font-weight: 400;\">The UMBI 2022 CRISPR workshop is tailored to give an overview of the Nobel-prize winning genome editing technology combined with several hands-on laboratory practical. This workshop consists of several lecture sessions that will comprehensively cover the important background and principal aspects of CRISPR technology. These include CRISPR initial discovery as the mechanism of bacteria adaptive immune response, CRISPR repurposing for eukaryotic gene targeting, CRISPR wide range of applications in the field of molecular biology and life sciences, and CRISPR genome editing experimental workflow. The participants will also get hands-on experience as well as tips and tricks from the experts focusing on the key laboratory techniques within this technology.<\/span><\/p>\n<p style=\"text-align: justify;\"><b>When?<\/b><\/p>\n<p style=\"text-align: justify;\"><span style=\"font-weight: 400;\">18-20<sup>th<\/sup> October 2022<br \/>\n<\/span><span style=\"font-weight: 400;\">8.30am \u2013 4.30pm<\/span><\/p>\n<p style=\"text-align: justify;\"><b>Where?<\/b><\/p>\n<p style=\"text-align: justify;\"><span style=\"font-weight: 400;\">集美麻豆 Medical Molecular Biology Institute (UMBI), Jalan Yaacob Latiff, 56000 Kuala Lumpur<\/span><\/p>\n<p style=\"text-align: justify;\"><b>Workshop fees<\/b><\/p>\n<p style=\"text-align: justify;\"><span style=\"font-weight: 400;\">RM 950 (Non-Student \u2013 Scientist, researcher, specialist, academicians, industry partners etc)<\/span><\/p>\n<p style=\"text-align: justify;\"><span style=\"font-weight: 400;\">RM 800 (Student)<\/span><\/p>\n<p style=\"text-align: justify;\"><span style=\"font-weight: 400;\">Fees include workshop materials and meals (3 times a day)<\/span><\/p>\n<p style=\"text-align: justify;\"><b>Why should you join?<\/b><\/p>\n<ul style=\"text-align: justify;\">\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Learn how to perform CRISPR genome editing experiment using the cost-effective conventional approach without completely relying on the commercial kits.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Facilitated by researchers who have extensive CRISPR genome editing with successful results and high-impact publications.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Opportunity to perform hands-on laboratory practical; choosing vector system, sgRNA designing and cloning, and delivering into cells. Important tips and troubleshooting strategies for a successful CRISPR experiment will also be shared.\u00a0<\/span><\/li>\n<li aria-level=\"1\"><span style=\"font-weight: 400;\">Free complete in-house CRISPR guidebook written by the facilitators<\/span><\/li>\n<\/ul>\n<p style=\"text-align: justify;\"><b>Who should attend?<\/b><\/p>\n<p style=\"text-align: justify;\"><span style=\"font-weight: 400;\">We welcome all Researchers, Scientists, Postgraduate Students, Application Specialists, or anyone who is interested in learning this CRISPR technology to attend. Although it is not essential, having basic molecular biology knowledge would be an advantage as the course will cover few lectures and practical sessions that are in intermediate-to-advanced levels. Nonetheless, we will accommodate all participants who are keen to join the workshop and learn this CRISPR genome editing technology.<\/span><\/p>\n<p style=\"text-align: justify;\"><span style=\"font-weight: 400;\">The workshop is limited to only <\/span><b>20 seats<\/b><\/p>\n<p style=\"text-align: justify;\"><b>Past workshop<\/b><\/p>\n<p style=\"text-align: justify;\"><span style=\"font-weight: 400;\">We had a successful inaugural CRISPR genome editing workshop that was held on the 18-20<sup>th<\/sup><\/span><span style=\"font-weight: 400;\"> November 2019 (Link: https:\/\/www.ukm.my\/umbi\/news\/crispr-gene-editing-workshop-2019\/). The workshop was attended by researchers and students from local and overseas institutions and has garnered several collaborations with participants who mainly come from the academia. In addition to the active collaboration with local and overseas researchers for CRISPR works, the facilitators have also been invited to give talks in several webinars and pre-conference workshops about this CRISPR genome editing technology.<\/span><\/p>\n<p><\/li><li class=\"tab-pane \" id=\"ert_pane1-2\"><\/p>\n<p><iframe src=\"https:\/\/docs.google.com\/forms\/d\/e\/1FAIpQLScgc0G80HebU-HL7a4f6lWhret0-Mt2xDmrMEAaRLnJO-f0WQ\/viewform?embedded=true\" width=\"100%\" height=\"1000\" frameborder=\"0\" marginwidth=\"0\" marginheight=\"0\"><\/iframe><\/p>\n<p><\/li><li class=\"tab-pane \" id=\"ert_pane1-3\"><\/p>\n<h3><strong>UMBI CRISPR Workshop (18-20 October 2022)<\/strong><\/h3>\n<p><strong>\u00a0<\/strong><\/p>\n<p><strong><em>Tuesday 18 October 2022 \u2013 Day 1<\/em><\/strong><\/p>\n<p><strong><em>\u00a0<\/em><\/strong><\/p>\n<p><strong><em>\u00a0<\/em><\/strong><\/p>\n<table style=\"height: 201px; border-color: black; margin-left: auto; margin-right: auto; width: 100%;\" border=\"1\" cellspacing=\"1\" cellpadding=\"1\">\n<tbody>\n<tr>\n<td width=\"113\"><strong>TIME<\/strong><\/td>\n<td colspan=\"2\" width=\"528\"><strong>PROGRAMME<\/strong><\/td>\n<\/tr>\n<tr>\n<td width=\"113\">0800-0900<\/td>\n<td width=\"520\"><strong>REGISTRATION AND BREAKFAST<\/strong><\/td>\n<td width=\"7\"><\/td>\n<\/tr>\n<tr>\n<td width=\"113\"><strong><em>\u00a0<\/em><\/strong><\/p>\n<p><strong><em>\u00a0<\/em><\/strong><\/p>\n<p><strong><em>\u00a0<\/em><\/strong><\/p>\n<p>0900<\/td>\n<td width=\"520\"><strong>\u00a0<\/strong><\/p>\n<p><strong>\u00a0Lecture 1 : Introduction and principle of CRISPR\/Cas9 technology<\/strong><\/p>\n<p><strong><em>\u00a0<\/em><\/strong><\/p>\n<p>\u00b7\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 Brief history<\/p>\n<p>\u00b7\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 Genome editing tools<\/p>\n<p>\u00b7\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 Principle of CRISPR-based genome editing<\/td>\n<td width=\"7\"><\/td>\n<\/tr>\n<tr>\n<td width=\"113\">1015<\/td>\n<td colspan=\"2\" width=\"528\"><strong>SHORT BREAK<\/strong><\/td>\n<\/tr>\n<tr>\n<td width=\"113\"><strong><em>\u00a0<\/em><\/strong><\/p>\n<p><strong><em>\u00a0<\/em><\/strong><\/p>\n<p><strong><em>\u00a0<\/em><\/strong><\/p>\n<p>1030<\/td>\n<td colspan=\"2\" width=\"528\"><strong><em>\u00a0<\/em><\/strong><\/p>\n<p><strong>Lecture 2: Cas9 variants and applications of CRISPR\/Cas9 technology<\/strong><\/p>\n<p><strong><em>\u00a0<\/em><\/strong><\/p>\n<p>\u00b7\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 Cas9 Nickase &amp; Dead Cas9<\/p>\n<p>\u00b7\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 Base Editing &amp; Prime Editing<\/p>\n<p>\u00b7\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 Research and Clinical Applications<\/p>\n<p>\u00b7\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 Ethical Issues<\/p>\n<p>&nbsp;<\/td>\n<\/tr>\n<tr>\n<td width=\"113\">1145<\/td>\n<td colspan=\"2\" width=\"528\"><strong>SHORT BREAK<\/strong><\/td>\n<\/tr>\n<tr>\n<td width=\"113\"><strong><em>\u00a0<\/em><\/strong><\/p>\n<p><strong><em>\u00a0<\/em><\/strong><\/p>\n<p><strong><em>\u00a0<\/em><\/strong><\/p>\n<p>1200<\/td>\n<td colspan=\"2\" width=\"528\"><strong><em>\u00a0<\/em><\/strong><\/p>\n<p><strong>Lecture 3: Starting your CRISPR experiment<\/strong><\/p>\n<p><strong>\u00a0<\/strong><\/p>\n<p>\u00b7\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 Developing Relevant Biological Questions<\/p>\n<p>\u00b7\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 General Gene Knockout and Knock-In Experimental Designs<\/p>\n<p>\u00b7\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 Choosing the Right CRISPR System<\/td>\n<\/tr>\n<tr>\n<td width=\"113\">1300-1415<\/td>\n<td colspan=\"2\" width=\"528\"><strong>LUNCH BREAK<\/strong><\/td>\n<\/tr>\n<tr>\n<td width=\"113\">1415<\/td>\n<td colspan=\"2\" width=\"528\"><strong>\u00a0Practical 1 \u2013 sgRNA design<\/strong><\/p>\n<p><strong>\u00a0<\/strong><\/p>\n<p>o\u00a0\u00a0\u00a0 Extracting genomic information (Ensembl Demo)<\/p>\n<p>o\u00a0\u00a0\u00a0 sgRNAs designing (CRISPick Demo)<\/p>\n<p>o\u00a0\u00a0\u00a0 sgRNAs target region visualization (Snapgene Viewer Demo)<\/p>\n<p>o\u00a0\u00a0\u00a0 Primer design to amplify targeted region<\/p>\n<p>&nbsp;<\/td>\n<\/tr>\n<tr>\n<td width=\"113\">1630<\/td>\n<td colspan=\"2\" width=\"528\"><strong>TEA &amp; END OF DAY 1<\/strong><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<p><strong><em>Wednesday 19 October 2022 \u2013 Day 2<\/em><\/strong><\/p>\n<p><strong><em>\u00a0<\/em><\/strong><br \/>\n&lt;t<\/p>\n<table style=\"height: 201px; border-color: black; margin-left: auto; margin-right: auto; width: 100%;\" border=\"1\" cellspacing=\"1\" cellpadding=\"1\">\n<tbody>\n<tr>\n<td width=\"113\"><strong>TIME<\/strong><\/td>\n<td colspan=\"2\" width=\"528\"><strong>PROGRAMME<\/strong><\/td>\n<\/tr>\n<tr>\n<td width=\"113\">0800-0900<\/td>\n<td width=\"520\"><strong>BREAKFAST<\/strong><\/td>\n<td width=\"7\"><\/td>\n<\/tr>\n<tr>\n<td width=\"113\"><strong><em>\u00a0<\/em><\/strong><\/p>\n<p><strong><em>\u00a0<\/em><\/strong><\/p>\n<p><strong><em>\u00a0<\/em><\/strong><\/p>\n<p>0900<\/td>\n<td width=\"520\"><strong>\u00a0<\/strong><\/p>\n<p><strong>\u00a0Lecture 4 : CRISPR-Cas9 experimental workflow<\/strong><\/p>\n<p><strong><em>\u00a0<\/em><\/strong><\/p>\n<p>\u00b7\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 Knockout\/Knockin workflow<\/p>\n<p>\u00b7\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 sgRNA cloning strategy<\/td>\n<td width=\"7\"><\/td>\n<\/tr>\n<tr>\n<td width=\"113\">1030<\/td>\n<td colspan=\"2\" width=\"528\"><strong>SHORT BREAK<\/strong><\/td>\n<\/tr>\n<tr>\n<td width=\"113\"><strong><em>\u00a0<\/em><\/strong><\/p>\n<p><strong><em>\u00a0<\/em><\/strong><\/p>\n<p><strong><em>\u00a0<\/em><\/strong><\/p>\n<p>1045<\/td>\n<td colspan=\"2\" width=\"528\"><strong><em>\u00a0<\/em><\/strong><\/p>\n<p><strong>Practical 2<\/strong><\/p>\n<p><strong>\u00a0<\/strong><\/p>\n<p>o\u00a0\u00a0\u00a0 Expression vector digestion<\/p>\n<p>o\u00a0\u00a0\u00a0 sgRNAs annealing<\/p>\n<p>o\u00a0\u00a0\u00a0 sgRNAs ligation<\/p>\n<p>o\u00a0\u00a0\u00a0 Bacteria transformation<\/p>\n<p>&nbsp;<\/td>\n<\/tr>\n<tr>\n<td width=\"113\">1300-1415<\/td>\n<td colspan=\"2\" width=\"528\"><strong>LUNCH BREAK<\/strong><\/td>\n<\/tr>\n<tr>\n<td width=\"113\"><strong><em>\u00a0<\/em><\/strong><\/p>\n<p><strong><em>\u00a0<\/em><\/strong><\/p>\n<p><strong><em>\u00a0<\/em><\/strong><\/p>\n<p>1415<\/td>\n<td colspan=\"2\" width=\"528\"><strong><em>\u00a0<\/em><\/strong><\/p>\n<p><strong>Lecture 5: Cells delivery and selection of positive cells<\/strong><\/p>\n<p><strong>\u00a0<\/strong><\/p>\n<p>\u00b7\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 CRISPR components delivery into the cells<\/p>\n<p>\u00b7\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 Determination of delivery efficiency<\/p>\n<p>\u00b7\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 Antibiotic selection (pooled cells isolation)<\/p>\n<p>\u00b7\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 Single cell sorting and dilution<\/p>\n<p>&nbsp;<\/td>\n<\/tr>\n<tr>\n<td width=\"113\">1515-1530<\/td>\n<td colspan=\"2\" width=\"528\"><strong>SHORT BREAK<\/strong><\/td>\n<\/tr>\n<tr>\n<td width=\"113\">1530<\/td>\n<td colspan=\"2\" width=\"528\"><strong>\u00a0<\/strong><\/p>\n<p><strong>Practical 3<\/strong><\/p>\n<p><strong>\u00a0<\/strong><\/p>\n<p>o\u00a0\u00a0\u00a0 CRISPR plasmids transfection<\/p>\n<p>o\u00a0\u00a0\u00a0 T7E1 endonuclease assay (PCR product rehybridization)<\/p>\n<p>&nbsp;<\/td>\n<\/tr>\n<tr>\n<td width=\"113\">1630<\/td>\n<td colspan=\"2\" width=\"528\"><strong>TEA &amp; END OF DAY 2<\/strong><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<p><strong><em>Thursday 20 October 2022 \u2013 Day 3<\/em><\/strong><\/p>\n<p><strong><em>\u00a0<\/em><\/strong><\/p>\n<table style=\"height: 201px; border-color: black; margin-left: auto; margin-right: auto; width: 100%;\" border=\"1\" cellspacing=\"1\" cellpadding=\"1\">\n<tbody>\n<tr>\n<td width=\"113\"><strong>TIME<\/strong><\/td>\n<td colspan=\"2\" width=\"528\"><strong>PROGRAMME<\/strong><\/td>\n<\/tr>\n<tr>\n<td width=\"113\">0800-0900<\/td>\n<td width=\"520\"><strong>BREAKFAST<\/strong><\/td>\n<td width=\"7\"><\/td>\n<\/tr>\n<tr>\n<td width=\"113\"><strong><em>\u00a0<\/em><\/strong><\/p>\n<p><strong><em>\u00a0<\/em><\/strong><\/p>\n<p>0900<\/td>\n<td width=\"520\"><strong>\u00a0<\/strong><\/p>\n<p><strong>\u00a0<\/strong><strong>Practical 4<\/strong><\/p>\n<p><strong>\u00a0<\/strong><\/p>\n<p>o\u00a0\u00a0\u00a0 Transfected cells visualization<\/p>\n<p>o\u00a0\u00a0\u00a0 T7E1 endonuclease assay (Digestion)<\/p>\n<p>&nbsp;<\/td>\n<td width=\"7\"><\/td>\n<\/tr>\n<tr>\n<td width=\"113\">1015-1030<\/td>\n<td colspan=\"2\" width=\"528\"><strong>SHORT BREAK<\/strong><\/td>\n<\/tr>\n<tr>\n<td width=\"113\"><strong><em>\u00a0<\/em><\/strong><\/p>\n<p><strong><em>\u00a0<\/em><\/strong><\/p>\n<p><strong><em>\u00a0<\/em><\/strong><\/p>\n<p>1030<\/td>\n<td colspan=\"2\" width=\"528\"><strong><em>\u00a0<\/em><\/strong><\/p>\n<p><strong>\u00a0Sponsored Talk : PLT Scientific<\/strong><\/p>\n<p><strong>\u00a0<\/strong><\/p>\n<p><strong>\u00a0Multiplex Western Blot Imaging and More with Azure Gel Imaging System<\/strong><\/p>\n<p>&nbsp;<\/td>\n<\/tr>\n<tr>\n<td width=\"113\">1130-1145<\/td>\n<td colspan=\"2\" width=\"528\"><strong>SHORT BREAK<\/strong><\/td>\n<\/tr>\n<tr>\n<td width=\"113\"><strong><em>\u00a0<\/em><\/strong><\/p>\n<p><strong><em>\u00a0<\/em><\/strong><\/p>\n<p>1145<\/td>\n<td colspan=\"2\" width=\"528\"><strong><em>\u00a0<\/em><\/strong><\/p>\n<p><strong>Practical 5<\/strong><\/p>\n<p><strong>\u00a0<\/strong><\/p>\n<p>o\u00a0\u00a0\u00a0 T7E1 endonuclease assay (Result discussion)<\/p>\n<p>o\u00a0\u00a0\u00a0 Azure Biosystem Demo (Western blot Imaging)<\/p>\n<p>&nbsp;<\/td>\n<\/tr>\n<tr>\n<td width=\"113\">1300-1415<\/td>\n<td colspan=\"2\" width=\"528\"><strong>LUNCH BREAK<\/strong><\/td>\n<\/tr>\n<tr>\n<td width=\"113\">1415<\/td>\n<td colspan=\"2\" width=\"528\"><strong>\u00a0<\/strong><\/p>\n<p><strong>Lecture 6: Characterization of edited cells and CRISPR-Cas9 case studies<\/strong><\/p>\n<p><strong>\u00a0<\/strong><\/p>\n<p><strong>Networking and Discussion <\/strong><\/p>\n<p>&nbsp;<\/td>\n<\/tr>\n<tr>\n<td width=\"113\">1630<\/td>\n<td colspan=\"2\" width=\"528\"><strong>TEA &amp; END OF WORKSHOP <\/strong><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<p>Link in PDF : <a href=\"https:\/\/www.ukm.my\/umbi\/wp-content\/uploads\/2022\/08\/UMBI-CRISPR-Workshop-2022-Tentative.pdf\">UMBI CRISPR Workshop 2022 Tentative<\/a><\/p>\n<p>&nbsp;<\/p>\n<p><\/li><li class=\"tab-pane \" id=\"ert_pane1-4\"><\/p>\n<p><span style=\"font-weight: 400;\">The CRISPR gene editing workshop will be held at 集美麻豆 Medical Molecular Biology Institute (UMBI), Kuala Lumpur that is located just a 15-minute drive away from the heart of Kuala Lumpur city center.<\/span><\/p>\n<p><span style=\"font-weight: 400;\">For more information about the venue, please visit the UMBI website at\u00a0<\/span><a href=\"https:\/\/www.ukm.my\/umbi\/\"><span style=\"font-weight: 400;\">https:\/\/www.ukm.my\/umbi\/.<\/span><\/a><\/p>\n<p><span style=\"font-weight: 400;\">Please take note that the accommodation is\u00a0<strong>NOT<\/strong>\u00a0provided. Kindly refer to the\u00a0Accommodation tab\u00a0for further information.<\/span><\/p>\n<p><b>Getting here:<\/b><\/p>\n<ul>\n<li><b>Public transport:\u00a0<\/b><\/li>\n<\/ul>\n<p style=\"font-weight: 400; padding-left: 26px;\">The nearest <strong>MRT<\/strong> station is Taman Pertama\/Taman Midah<br \/>\nThe nearest <strong>LRT<\/strong> stations is Salak Selatan\/Bandar Tun Razak<br \/>\nThen take a GRAB or taxi to UMBI.<\/p>\n<ul>\n<li><b>By Car:<\/b><\/li>\n<\/ul>\n<p style=\"font-weight: 400; padding-left: 26px;\">Participant can park at Zone L parking in the 集美麻豆 Medical Center (see attached map)<\/p>\n<p>&nbsp;<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-97086 aligncenter\" src=\"https:\/\/www.ukm.my\/umbi\/wp-content\/uploads\/2022\/06\/Cripsr2022_map.png\" alt=\"\" width=\"701\" height=\"434\" srcset=\"https:\/\/www.ukm.my\/umbi\/wp-content\/uploads\/2022\/06\/Cripsr2022_map.png 512w, https:\/\/www.ukm.my\/umbi\/wp-content\/uploads\/2022\/06\/Cripsr2022_map-300x186.png 300w\" sizes=\"(max-width: 701px) 100vw, 701px\" \/><\/p>\n<p>&nbsp;<\/p>\n<p><span style=\"font-weight: 400;\">WAZE:\u00a0<\/span><a href=\"https:\/\/www.waze.com\/directions\/malaysia\/kuala-lumpur\/umbi-ukm\/66650143.666632502.6392182.html\"><span style=\"font-weight: 400;\">https:\/\/www.waze.com\/directions\/malaysia\/kuala-lumpur\/umbi-ukm\/66650143.666632502.6392182.html<\/span><\/a><\/p>\n<p><span style=\"font-weight: 400;\">GOOGLE MAP:\u00a0<\/span><a href=\"https:\/\/goo.gl\/maps\/soeAgH9K4292\"><span style=\"font-weight: 400;\">https:\/\/goo.gl\/maps\/soeAgH9K4292<\/span><\/a><\/p>\n<p><\/li><li class=\"tab-pane \" id=\"ert_pane1-5\"><\/p>\n<p><span style=\"font-weight: 400;\">Hotels nearby UMBI:<\/span><\/p>\n<ul>\n<li><span style=\"font-weight: 400;\">Hotel Sri Permaisuri Cheras (3-star hotel)<\/span><\/li>\n<li><span style=\"font-weight: 400;\">Hotel EST Kuala Lumpur<\/span><\/li>\n<li><span style=\"font-weight: 400;\">International Youth Centre (IYC) Residence<\/span><\/li>\n<li><span style=\"font-weight: 400;\">Hotel Maple Inn<\/span><\/li>\n<li><span style=\"font-weight: 400;\">V Hotel Kuala Lumpur<\/span><\/li>\n<li><span style=\"font-weight: 400;\">Crystal Garden Hotel<\/span><\/li>\n<\/ul>\n<p><span style=\"font-weight: 400;\">Low Budget hotels are also available nearby the UMBI. Please refer to any hotel booking website for further information.<\/span><\/p>\n<p><span style=\"font-weight: 400;\">**Important Note: KINDLY BE NOTED THAT THE ORGANIZER WILL NOT BE LIABLE FOR ANY CLAIM OR RESPONSIBILITY IN REGARDS OF THE LISTED ACCOMMODATION ABOVE. FOR ACCOMMODATION BOOKING AND RESERVATION, PLEASE CLICK THE APPROPRIATE LINKS AND FOLLOW THE GUIDELINES IN THE RESPECTIVE WEBSITE.<\/span><\/p>\n<p><\/li><li class=\"tab-pane \" id=\"ert_pane1-6\"><\/p>\n<p>Contact details:<\/p>\n<p><strong>Miss Siti Nur Hasanah: +603 9145 9087 \/ 9185<\/strong>\u00a0 (email: <a href=\"mailto:hasanahyusuf@ukm.edu.my\">hasanahyusuf@ukm.edu.my<\/a>)<br \/>\n集美麻豆 Medical Molecular Biology Institute (UMBI),<br \/>\nJalan Yaacob Latiff, Bandar Tun Razak,<br \/>\n56000 Cheras, Kuala Lumpur, Malaysia<\/p>\n<p><\/li><\/ul><\/div><\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":1001007,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":""},"class_list":["post-97068","page","type-page","status-publish","hentry"],"acf":[],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/www.ukm.my\/umbi\/wp-json\/wp\/v2\/pages\/97068","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.ukm.my\/umbi\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.ukm.my\/umbi\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.ukm.my\/umbi\/wp-json\/wp\/v2\/users\/1001007"}],"replies":[{"embeddable":true,"href":"https:\/\/www.ukm.my\/umbi\/wp-json\/wp\/v2\/comments?post=97068"}],"version-history":[{"count":85,"href":"https:\/\/www.ukm.my\/umbi\/wp-json\/wp\/v2\/pages\/97068\/revisions"}],"predecessor-version":[{"id":97609,"href":"https:\/\/www.ukm.my\/umbi\/wp-json\/wp\/v2\/pages\/97068\/revisions\/97609"}],"wp:attachment":[{"href":"https:\/\/www.ukm.my\/umbi\/wp-json\/wp\/v2\/media?parent=97068"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}